Pam Young, Indiana University School of Medicine

Practical Limitations in Multiphoton Microscopy

Multiphoton microscopy (MPM) has become widely used in biomedical research. Unfortunately, imaging depth in MPM is limited. Fluorescence signals attenuate with depth due to light scattering and absorption in the tissue as well as from refractive index mismatch. Even in a homogenous sample, there can be refractive index mismatch between the objective lens, immersion medium, coverslip, and sample which causes the focal point to broaden with depth.

LOCI Biomedical Engineering Series Seminar- Hao Zhang, University of Wisconsin at Milwaukee

Multimodal ophthalmic imaging, Prof. Hao Zhang

Diabetic retinopathy (DR), a retinal disease characterized by microvascular damage to the retina in patients with diabetes, is a leading cause of irreversible blindness among working-age adults. DR progresses through a sequence of recognizable stages, which begin with structural and functional derangement of the retinal microcirculation even before the early clinical signs occur.

LOCI Biomedical Engineering Series Seminar- Andy Cohen, University of Wisconsin at Milwaukee

My research involves developing generally applicable theoretical approaches and software tools that enable quantification, discovery and hypothesis testing on complex biological processes. This disease-oriented research uses techniques from algorithmic information theory and algorithmic statistics, image processing and multi-target tracking, machine learning, and high performance computing to analyze sequences of images of live cells and tissue. This research is capable of outperforming the human eye in detecting subtle differences in dynamic behaviors.