|Instrument Sort descending||Name/Model #|
|Bock Prairie Laser Scanning Confocal
This three-channel laser scanning confocal is designed on a Nikon Eclipse Ti inverted microscope as a modular system based on a core galvanometer imaging module. Researchers can customize their experiments with an automated 9-pinhole aperture (pinhole size ranging from 30 mm – 2000 mm), filter wheels for each of the three detection channels, and 4-posit
|Bock Prairie Laser Scanning Confocal|
|Compact Automated Multiphoton Microscope
CAMM is an inverted multiphoton laser scanning system best suited for histological samples for coregistration of large field-of-view forward detected second harmonic generation photons and brightfield built on an ASI RAMM frame that uses a tunable CAMM
|Dual head teaching histology microscope
The dual-head teaching microscope is an upright microscope located in the main LOCI office for histological purposes.
The objectives available on the microscope are:
|GFP Dissecting Stereo Microscope|
LINK is an instrumentation collaboration between the Medical Engineering group in the Morgridge Institute for Research and LOCI investigators that combines quantitative ultrasound (QUS), forwards and backwards scattered second harmonic generation microscopy (SHG), and two optical scattering techniques, enhance backscatter and optical coherence tomography (EBS and OCT, respectively) to analyze collagen organization in intact animal and
The Opterra Multipoint Scanning Confocal Microscope is being developed in collaboration with LOCI for improvements in speed, software analysis, photomanipulation and fast spectral collection.
This is a custom built multiphoton laser scanning system built around a Nikon TE300 inverted microscope. The system features dual galvos for simultaneous imaging and photomanipulation, three lasers and time-domain fluorescence lifetime.
The OWS is the system most often used by collaborators. Experiments often run on the OWS include collagen imaging of tumor sections using second harmonic generation, lifetime imaging, and imaging of autofluorescent molecules such as NADH.
|Optical Workstation II
OWS II is an inverted multiphoton laser scanning instrumentation development platform with backscattered second harmonic generation (SHG) and epifluorescence capabilities that uses a Nikon Eclipse Ti-U inverted base equipped with an epifluorescent excitation lamp, OWS II
Spectral lifetime imaging microscopy (SLIM) simultaneously measures fluorescence lifetime and spectra for intrinsic and extrinsic fluorophores. This technique discriminates between multiple fluorophores and provides information about the microenvironment – ionic strength, bound state, oxygen concentration, etc. SLIM provides a “fingerprint” of a fluorophore allowing more accurate tracking by researchers, and fingerprints of intrinsic fluorophores such as NADH can provide metabolic maps of cells.
The Bruker Ultima IntraVital is a four-channel multiphoton laser scanning microscope with second harmonic generation (SHG) capabilities with a especially designed for imaging living things. and up to thirteen inches of space beneath the objective to allow live imaging of medium-sized organisms.
Ultra is a multiphoton long-wavelength scanning microscope system under development by LOCI. It is a hybrid design of fabrication systems including the Fluoview imaging system and uses Wiscan imaging software. Forward detection can be used to measure second harmonic generation (SHG) and third harmonic generation (THG). The system will also have adaptive optics and lifetime measurement capabilities. The OPO System will have a wide range of uses including broadband excitation, label-free imaging, polarization control, and deep imaging. The laser source is a
|Zeiss Inverted Tissue Culture Fluorescence Microscope
Zeiss Axiovert 25 CFL inverted light microscope for routine analyses of cell and tissue cultures in transmitted and fluorescent reflected light.
The fluorescence setup has the following filter sets:
FL Filter Set 02, --example fluorophore DAPI
Zeiss filter set 02 excites at 365 nm. and allows emission at 420 nm.