Instrument Sort descending Name/Model #
Boom Dissecting Stereo Microscope
Bruker Ultima in vivo multiphoton microscope

The Bruker Ultima IV is specifically designed for in vivo research but can be used for a variety of multiphoton applications.  The stage is separate from the microscope setup and can be removed to allow for additional clearance under the lenses; up to 13 inches of space under the objective lens allows for visualization of medium-sized organisms.  A brightfield light can be used to locate an area of interest, and a substage detector allows for direction-dependent polarization experiments.

Ultima IV
Dual head teaching histology microscope

 

The Dual Head Teaching Scope is an upright microscope located in the main LOCI office, and it is especially useful for histology. 

The objectives available on the microscope are:

BX53
GFP Dissecting Stereo Microscope
LINK

LINK is an instrumentation collaboration between the Medical Engineering group in the Morgridge Institute for Research and investigators in the UW Laboratory for Optical and Computational Instrumentation (LOCI).

LINK
LOCI confocal at BOCK

 

This Multi-purpose Three Channel Confocal was designed and built by Prairie Technologies on a Nikon Eclipse Ti inverted microscope. The confocal microscope is a modular system based on the core galvanometer imaging module found in other Prairie confocals.

Multi-purpose Three Channel Confocal
LOCI confocal development system

 

Leica SP2 AOBS system mounted on DMIRE2 inverted microscope.  Multiple laser lines allows for excitation of a wide range of fluorphores, and simultaneous multi-spectral scanning is possible.  The microscope also employs a galvanometer z-stage, transmitted light detector and manual condenser with DIC optics.

SP2 AOBS
Opterra Multipoint Scanning Confocal Microscope

Spectral Swept-Field Confocal

 

Opterra
Optical workstation (OWS) multiphoton

 

This is a custom built multiphoton laser scanning system built around a Nikon TE300 inverted microscope. The system features dual galvos for simultaneous imaging and photomanipulation, three lasers and time-domain fluorescence lifetime. 

The OWS is the system most often used by collaborators.  Experiments often run on the OWS include collagen imaging of tumor sections using second harmonic generation, lifetime imaging, and imaging of autofluorescent molecules such as NADH.

OWS
OptiMiS multiphoton microscope

 

Under a UW-Milwaukee and UW-Madison Biophotonics partnership the Raicu group is developing the OptiMiS multiphoton microscope. This is a novel multiphoton microscopy with spectral resolution for with spectral resolution forr spectrally resolving heterogeneous signals in live cell imaging. A specific focus is fluorescence resonance energy transfer (FRET) studies.

OptiMiS
Spectral lifetime multiphoton microscope (SLIM)

 

Spectral lifetime imaging microscopy (SLIM) simultaneously measures fluorescence lifetime and spectra for intrinsic and extrinsic fluorophores.  This technique can discriminate between multiple fluorophores and provide information about the microenvironment.

The SLIM system at LOCI is fitted on a Nikon Eclipse TE2000U base.  An excitation laser is tunable from approximately 700-980 nm.  Three detectors allow for recording of emitted fluorescence.

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Spectral Lifetime Imaging Microscope (SLIM)
Ultra

Ultra is a multiphoton long-wavelength scanning microscope system under development by LOCI.  It is a hybrid design of fabrication systems including the Fluoview imaging system and uses Wiscan imaging software.  Forward detection can be used to measure second harmonic generation (SHG) and third harmonic generation (THG).  The system will also have adaptive optics and lifetime measurement capabilities.  The OPO System will have a wide range of uses including broadband excitation, label-free imaging, polarization control, and deep imaging. The laser source is a

Zeiss Inverted Tissue Culture Fluorescence Microscope

Zeiss Axiovert 25 CFL inverted light microscope for routine analyses of cell and tissue cultures in transmitted and fluorescent reflected light.

 The fluorescence setup has the following filter sets:

FL Filter Set 02, --example fluorophore DAPI

Zeiss filter set 02 excites at 365 nm. and allows emission at 420 nm.

Axiovert 25