This Multi-purpose Three Channel Confocal was designed and built by Prairie Technologies on a Nikon Eclipse Ti inverted microscope. The confocal microscope is a modular system based on the core galvanometer imaging module found in other Prairie confocals.
Researchers can customize their experiments with an automated 9-pinhole aperture (pinhole size ranging from 30 mm – 2000 mm), filter wheels for each of the three detection channels, and 4-position automated dichroic holders. This system has a photoactivation module along with uncaging/photoactivation optics for simultaneous imaging and photoactivation/uncaging experiments. The motorized stage allows for the automated collection of data at multiple sample locations. This confocal is also capable of reflectance confocal imaging with the 515 nm laser.