This is a custom built multiphoton laser scanning system built around a Nikon TE300 inverted microscope. The system features dual galvos for simultaneous imaging and photomanipulation, three lasers and time-domain fluorescence lifetime.
The OWS is the system most often used by collaborators. Experiments often run on the OWS include collagen imaging of tumor sections using second harmonic generation, lifetime imaging, and imaging of autofluorescent molecules such as NADH.
- LSI VSL 337 ND-6 Ablation laser (with DLM 220). Nitrogen-pumped dye laser for laser microsurgery or UV photolysis. Used with Coumarin 450 laser dye
- Amplitude Systems T-Pulse 1027 fixed wavelength laser
- BioRad MRC-1024 photodiode transmission detector
- ASI MS-2000 XYZ stage
- Becker-Hickl SPC-830 Time-Correlated Single Photon Counting Board
- controlled by WiscScan
Semrock 750 nm (SP) transmission scan
Semrock 450/70 nm transmission scan