|37 Non-CO2 Incubator|
|BioClave Benchtop Autoclave||
BioClave 16 benchtop steam sterilizer has a chamber measuring 9" diameter x 13ó" deep. Steam sterilization of research tools and consumables occurs in a stainless steel sterilization chamber with a electrical safety interlock that prevents the door from being opened until the pressure has reached zero PSI. Three trays are included, along with a conventional tray removal tool. With large digital displays and fully automatic operation, all segments of the sterilization cycle (fill, sterilize, exhaust and dry) commence and run to completion by simply pressing the start button. The 16 liter BioClave offers three cycle options (standard programs), which are preset for the sterilization of liquids, wrapped instruments or unwrapped instruments/plasticware. A fourth option "Dry Only" is also available for additional drying time to be added to the end of a cycle. For special applications, requiring variations in cycle parameters, all settings on the BioClave 16 are fully adjustable.
|Compact automated multiphoton microscope||
Compact Automated Multiphoton Microscope, abbreviated “CAMM,” is a combined multiphoton and white light slide scanning system that has been designed to screen samples for tumor associated collagen signatures (TACS). Samples are automatically scanned over many fields of view using our custom laser scanning system WiscScan integrated with a bare bones microscope frame from ASI. The extended field of view multiphoton and white light images are automatically stitched and registered with each other using the open source image analysis software FIJI/ImageJ. We are then able to perform automated TACS scoring on the sample using our CurveAlign software tool.
To automatically screen samples for tumor associated collagen signatures.
The purpose of the diagram above was to help in the opto/mechanical design of the system.
CAMM Lens Inventory:
Plan N 4x/.10
Nikon Plan Apo 20X/0.75
|Fluorescence Lifetime Imaging (FLIM)||
|Gel Camera System|
|Horiba FluoroLog Spectrophotometer||
The Fluorolog-3-22 (JY Horiba Inc.) is a spectrofluorometer consisting of a 450 W xenon arc lamp, a double excitation monochromator, a sample compartment with a cuvette holder, a double emission monochromator, and a photomultiplier tube (PMT). A fiber-optic probe can be coupled to the sample compartment such that spectroscopic measurements can be made remotely. Although the spectrofluorometer has been designed for fluorescence spectroscopy, fluorescence (as either an excitation or emission spectrum) as well as reflectance spectra (in synchronous scan mode) can be measured. The adjustable variables are excitation wavelength(s) and emission wavelength(s), excitation and emission slit widths, sampling increment, integration time, and collection geometry (front face versus right angle collection).
Imaris is a scientific software program designed by Bitplane that allows for visualization, analysis, and interpretation of microscopy data. Imaris can be used to analyze both large (50 GB or more) 3D and 4D multi-channel images.
The program is designed for the life sciences and can decrease the amount of time spent interpreting and optimizing data. Large data sets can be segmented to identify and visualize individual ojects, and Imaris MeasurementPro can be used to obtain statistical information.
|Minus 80 Freezer|
The following Nikon objectives are available:
The following Olympus objectives are available:
The following Zeiss objectives are available:
|Polymerase Chain Reaction (PCR) Machine|
|Sequential erosive tissue imaging (SETI)||
Sequential Erosive Tissue Imaging, abbreviated “SETI,” is a block face imaging system that combines a precision milling machine with epi-fluorescence and reflected white light microscopy. Samples are embedded in opaque, hard plastic resin or flash frozen in tissue freezing media. Tissue blocks are then mounted and aligned onto the SETI x-y translation stage. The milling system removes a 2.5 micron layer off the top of the tissue block and any debris is cleared by a compressed air blower/Hepa2 vacuum system. Stitched epi-fluorescence and white light images are captured at each newly revealed layer producing 2.5 micron resolution images of the entire 2D cross section. The process is repeated in an automated fashion until the sample is completely digitally captured. High resolution 3D images are then stitched and rendered using the open source image analysis software FIJI/ImageJ.
Enable automated serial section reconstruction allowing for convenient 3D gene expression studies or high resolution 3D anatomical mapping.
|Tissue Culture Hood||
Class II, Type A2 Biological Safety Cabinet, Vertical Laminar Flow Bench
|Triax 320 Spectrograph||
The system consists of a 450 W xenon lamp (FL-1039, J.Y. Horiba) as the broad band light source, a scanning double excitation monochromator (Gemini 180, J.Y. Horiba) to obtain a series of monochromatic excitation light, a bifurcated fiber-optic probe (multimode fibers) for light delivery, a filter wheel, an imaging spectrograph (Triax 320, J.Y. Horiba) and a CCD camera (CCD3000, J.Y. Horiba) for detection. With this system, point measurement of fluorescence and diffuse reflectance can be performed on the surface of tissues.