Prairie Ultima Intravital Multiphoton

Basic Information
Manufacturer: 
Prairie Technologies
Name/Model #: 
Prairie Ultima IV
Type: 
Multiphoton Microscope for In Vivo Research
Description: 

 

The IV Multiphoton was built by Prairie Technologies in Middleton, WI.  It is especially useful for in vivo research but can be used for a variety of multiphoton applications.  The stage is separate from the microscope setup and can be removed to allow for additional clearance under the lenses; up to 13 inches of space under the objective lens allows for visualization of medium-sized organisms.  A brightfield light can be used to locate an area of interest, and a substage detector allows for direction-dependent polarization experiments.

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Advanced Information
Excitation Sources: 
2P Laser, Brightfield
Objectives (Lenses): 
10x 0.3NA UPlanFL N Air WD 10mm (Olympus)
10x 0.5NA SFlour Air WD 1.2mm (Nikon)
20x 0.5NA UPlanFL Air WD 2.1mm (Olympus)
20x 1.0NA Plan-Apochromat Water WD 1.7 (Zeiss)
40x 0.75NA UPlanFL Air WD 0.51mm (Olympus)
40x 0.8NA LUMPlanFN N Water WD 3.3mm (Olympus)
40x 1.0NA Plan-Apochromat Water WD 2.5mm (Zeiss)
60x 1.2NA UPlanSApo Water WD 0.28mm (Olympus)
60x 1.4NA PlanApo Oil WD 0.15mm (Olympus)
Filters: 
Filter Cubes
Detectors: 
Configurable for 1 to 4 hand-picked Hamamatsu Multi-alkali PMTs
Other Features: 
  • Variable height XY stage, minimum 0.3 um increments
  • Z-motor, 25 mm travel, 0.1 um step
  • Prairie View imaging software - For the Prairie View manual, please visit the Prairie website here.
Location Information
Location: 
Animal Sciences, 1675 Observatory Drive, Madison, WI 53706
Access Rules: 

LOCI Policy

See Also
Manufacturer's Ultima IV page
Manufacturer's Prairie View manuals
PreviewAttachmentSize
Start Up Procedure for Ultima.doc187.5 KB
Prairie View Manual for Ultima.doc44 KB
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Related Images

Prairie Intravital picture no cap
In vivo image of human breast cancer cells in a mouse mammary gland surrounded by collagen.