Spectral lifetime imaging microscopy (SLIM) simultaneously
measures fluorescence lifetime and spectra for intrinsic and extrinsic
fluorophores. This technique can discriminate between multiple fluorophores and provide
information about the microenvironment.
The SLIM system at LOCI is fitted on a Nikon Eclipse TE2000U
base. An excitation laser is
tunable from approximately 700-980 nm.
Three detectors allow for recording of emitted fluorescence.