Spectral Lifetime Multiphoton Microscope

Basic Information
Name/Model #: 
Spectral Lifetime Imaging Microscope (SLIM)
Type: 
Spectral Lifetime Multiphoton Microscope
Description: 

 

Spectral lifetime imaging microscopy (SLIM) simultaneously measures fluorescence lifetime and spectra for intrinsic and extrinsic fluorophores.  This technique can discriminate between multiple fluorophores and provide information about the microenvironment.

The SLIM system at LOCI is fitted on a Nikon Eclipse TE2000U base.  An excitation laser is tunable from approximately 700-980 nm.  Three detectors allow for recording of emitted fluorescence.

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Advanced Information
Excitation Sources: 
Coherent MIRA 900 tunable excitation laser
Objectives (Lenses): 
Nikon Objectives
Other Features: 

Detectors:

  • Becker-Hickl PML-16
  • Hamamatsu H7422
  • Hamamatsu MCP
Location Information
Location: 
Animal Sciences, 1675 Observatory Drive, Madison, WI 53706
Access Rules: 

LOCI Policy

PreviewAttachmentSize
SLIM explanation.pdf3.51 MB
SLIM applications.pdf378.78 KB
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Related Images

SLIM image
SLIM images of live MCF10 breast cancer cells. Panels depict images at (a)420, (b)440, (c)470, and (d)510 nm. Cells were excited at 780 nm. BioTechniques (2006) 41: 249.