Spectral lifetime imaging microscopy (SLIM) simultaneously measures fluorescence lifetime and spectra for intrinsic and extrinsic fluorophores. This technique can discriminate between multiple fluorophores and provide information about the microenvironment.
The SLIM system at LOCI is fitted on a Nikon Eclipse TE2000U base. An excitation laser is tunable from approximately 700-980 nm. Three detectors allow for recording of emitted fluorescence.