Comstat2 is a Java-based computer program for the analysis and treatment of biofilm images in 3D. It is the Master's project of Martin Vorregaard.

Comstat2 uses the Bio-Formats Importer plugin for ImageJ to read files in TIFF and Leica LIF formats.

For more information, see the IMM paper:

Comstat2 - a modern 3D image analysis environment for biofilms