New approaches for live cell and organelle microscopy with Andrew Cohen

Tracking is just the beginning: new approaches for live cell and organelle microscopy
Andrew R. Cohen
Electrical & Computer engineering, Drexel University 

Event Date
September 21, 2017 11:00am
Room 274 Animal Sciences

Live cell and tissue microscopy generates multichannel 2-D and 3-D images capturing dynamic processes of disease and development. To make best use of these movies requires software tools for analysis, visualization and interaction. This talk will describe new algorithms for analyzing time-lapse microscopy image sequences, and new software tools called leverjs for visualizing and interacting with the image data together with the analysis results. The program includes a zero-error general-purpose tracking engine, combined with a new storage architecture available from any image analysis environment. The system can be run stand-alone, or as a web-based client/server providing a machine/crowd-source framework for high-throughput annotation of images and analysis. Hardware accelerated 2-D and 3-D multichannel rendering is done in the browser using a new custom raycasting engine. Comprehensive security options are available for the web-based application. All of the software will be available free and open source, and we are eager to find ways to integrate the program into new platforms and applications. The talk will showcase movies generated with the software, and will demonstrate the ability to interact with and visualize image data and analysis results from any web browser, with applications including 2-D and 3-D human and mouse stem and tumor cells, mitochondrial tracking and analysis, and 6 channel 3-D organelle movies.

Andrew Cohen joined the faculty in the department of electrical and computer engineering at Drexel University as an associate professor in August 2012. Before coming to Drexel, he was an assistant professor at the University of Wisconsin-Milwaukee. He received his Ph.D. from the Rensselaer Polytechnic Institute in May 2008 working with Prof. Badri Roysam. His postdoctoral research on developing computational approaches to quantifying deficiencies in axonal organelle transport due to neurodegenerative disease was funded by the Huntington's Disease Foundation. Dr. Cohen was previously employed as a software design engineer in the DirectX group at Microsoft where he designed operating systems software for gaming applications and as a microprocessor product engineer at Intel Corp. Dr. Cohen is a senior member of the IEEE.