This page describes LOCI's role in developing SLIM Curve. For the main web site, see:
SLIM Curve is an exponential curve fitting library used for Fluorescent Lifetime Imaging (FLIM) and Spectral Lifetime Imaging (SLIM). It is developed by Paul Barber and the Advanced Technology Group at the Cancer Research UK and Medical Research Council Oxford Institute for Radiation Oncology at the University of Oxford, as well as the Laboratory for Optical and Computational Instrumentation at the University of Wisconsin-Madison. SLIM Curve is used for FLIM functionality in the Advanced Technology Group's Time Resolved Imaging (TRI2) software, as well as in the SLIM Curve plugin for ImageJ.
LOCI became increasingly interested in lifetime (FLIM) and combined spectral lifetime (SLIM) image analysis after constructing a prototype SLIM microscope system in-house in 2005 using Becker & Hickl hardware. In 2006, we created a proof-of-concept software application known as SLIM Plotter to analyze data from this system, with the idea to eventually integrate the code into our VisBio application. However, over time it became increasingly clear that fluorescence lifetime analysis is a complex endeavor which would benefit from collaboration with experts outside our group. We became aware of the TRI2 project created by Paul Barber, and teamed up with him to create a shared open source codebase for FLIM and SLIM analysis: SLIM Curve. As a prerequisite to the project, we put substantial effort into removing all uses of Numerical Recipes algorithms from the SLIM Curve codebase, freeing it from any potential licensing issues. Since then, we have been developing an ImageJ plugin for FLIM and SLIM analysis built on the SLIM Curve library.
As an aside, the SLIM Curve efforts have also been a driving use case in some of the architectural elements of the ImageJ2 and SCIFIO projects, particularly support for image data beyond the usual five dimensions of X, Y, Z, channels and time.