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The Optical Workstation II is an open source laser scanning microscope development platform for multiphoton, SHG, and epifluorescence imaging including hardware and software
Multiphoton Laser Scanning, Backscattered SHG, Epifluorescence

OWS II is an inverted multiphoton laser scanning instrumentation development platform with backscattered second harmonic generation (SHG) and epifluorescence capabilities that uses a Nikon Eclipse Ti-U inverted base equipped with an epifluorescent excitation lamp, Spectra-Physics Tsunami Model 3941-X1BB (S/N 1561G) Tsunami Series Ti-Saph laser and a Millennia Pro s-Series Spectra Physics (S/N E2362) pump, and Hamamatsu H7422P-40 PMT detector with high sensitivity in the 300 to 720 nm wavelengths.  OWS II uses a Cambridge Technology 6210H galvonometer scanner +/-15 mechanical degrees, +/-30 optical degrees with a 671XX servo driver and 3mm XY mirror set.  The hardware control unit for OWS II can be integrated with multiple imaging modalities and uses a NI FGPA integrated circuit for intrinsic hardware parallelism and timing for greater flexibility and interoperability than standard digital signal processors (DSPs) -- an adaption that gives the software developer more freedom to control both software and hardware.  OWS II uses a Conoptics Model 302 RPM (S/N 5009) modulator to control light intensity, halfplate/quarterplate polarization filters to generate circularly polarized light for backscattered detected second harmonic generation (SHG) a Uniblitz VMM D4 shutter driver to control four shutters independently and/or simultaneously. The OWS II epifluorescent system uses a Diagnostic Instruments Model 18.3 Three Shot Color (S/N 249825) camera and a Prior Scientific Lumen 200 Pro epifluorescence illumination system.  A 45-degree mirror mounted on a motorized ThorLabs NRT100 stage is used to switch the system between laser scanning and epifluorescence.  The sample is held on a X-Y-Z stage controlled by an Applied Scientific Instruments MS-2000 stage controller for automated data acquisition.  The microscope is further equipped with 10X and 20X objectives and a motorized Sutter Instrument filter wheel.

Output Characteristics                Tsunami Broadband fs 10W Pump
Tuning Range 700-1000 nm
Average Power >1.4 W at 800 nm
Pulse Width <100 fs
Peak Power >170 kW at 800 nm
Pulse Energy ~14 nJ

Class IV Laser: Safety goggles for OWSII are located in room 158 in the metal shelf next to the door.  There are two pairs: a pair from GPT offering protection from 190-400 nm light as well as 840-1070 nm light, and a pair from Amazon offering protection from 740-1100 nm of light. OWS II uses a Class IV Spectra-Physics Millennia-Pro (SN#E2362) diode-pumped solid-state CW laser and a Class IV Spectra-Physics Tsunami (SN#1561G) Ti:Sapph pulsed laser.

Scientists and Staff
Bing Dai (shown above with the OWS II) is the lead scientist at LOCI for developing hardware control solutions for OWS II and lead developer of the in-house laser scanning program for improved functionality. Rui Xu assists Bing with OWS II optical alignment and LabVIEW FPGA/C++ coding for fundamental hardware drivers for digital waveform generation, line/pixel clock generation, and data acquisition (photon counting).

Bing DaiRui Xu
Bing Dai                                        Rui Xu

OWS II Instrumentation Development Book Pages
ASI Imaging Z motor
Operating instructions for OWS II
Conoptics Pockels Cell Alignment Notes
Objective inventory

How to change and clean objective lenses
How to remove OWS II filter wheel

Custom Adapter: ThorLabs RP01 Rotation Stage to Cambridge Technology D03806 REV B1 Galvo Mount

Breast tissue image taken on OWS IIBreast Tissue

Breast tissue images taken on OWS II.

Manuals and Technical Specifications