See the Coverslips page for information on coverslips.
See the Objective Specifications page for details on objectives.
The following websites can be used to find spectral information for various fluorophores.
High magnification non-immersion objectives are designed to be used with air between the front lens and the cover glass. Most objectives are designed to used with a standard cover glass thickness of 0.17 millimeters (a number 1.5 cover glass). When air objectives with high numerical apertures (0.8 or greater) are used, variations of just a few micometers in cover glass thickness can