Compact Automated Multiphoton Microscope

Name/Model #
Multiphoton Laser Scanning, Large Field-of-View Forward SHG and Brightfield
271 Animal Sciences 1675 Observatory Drive, Madison, WI, 53706

CAMM is an inverted multiphoton laser scanning system best suited for histological samples for coregistration of large field-of-view forward detected second harmonic generation photons and brightfield built on an ASI RAMM frame that uses a tunable Coherent MIRA900 laser excitation source (S/N 96994) for imaging from 700-1000 nm pumped by a Coherent VerdiV10 (S/N V10-G9920) with XY scanning control by Cambridge 6210 galvos.  CAMM excitation is modulated by a Conoptics 350-80-LA Pockels cell (S/N 353273BK) with a Hamamatsu H7422-40P detector to collect forward scattered SHG photons via the 780 nm tuned excitation source used in conjunction with a ThorLabs MF390-18 excitation filter. CAMM's brightfield scanning system is equipped with a 01-QIClick-F-CLR-12 Color 12 BIT CCD Camera (S/N Q29664) to screen histological samples for tumor associated collagen signatures (TACS) and an ASI Continuous Reflection Interface Sampling and Positioning (CRISP) system (S/N 8) for continuous focus stability and reduced hourly drift <0.1μm for longer scans. The extended field-of-view multiphoton and white light images automatically stitch and register using the open source image analysis software FIJI/ImageJ for automated TACS scoring on the sample using the open source CurveAlign software tool developed by LOCI lead scientist Yuming Liu.

Class IV Laser: Safety goggles for CAMM are located in room 159B on the hook on the wall offering protection for 740-1100 nm light. CAMM uses a Class IV Coherent Mira 900 (#SN 96994) Ti:Sapph pulsed laser and a Class IV Coherent Verdi-V10 (#SN V10-G9920) diode-pumped solid-state CW laser.  Engineers may wish to refer to the American National Standard for Safe Use of Lasers and follow LOCI's General Laser Safety Guidelines.

Coregistration of forward second harmonic generation on CAMM

Healthy human breast tissue imaged by Matt Conklin with 40x objective on CAMM with brightfield (center) and forward detected second harmonic generation image of collagen  (left, higher magnification right).

Scientists and Staff

Adib Keikhosravi
Adib Keikhosravi manages CAMM collaborations and is developing image registration and segmentation code in MATLAB for CurveAlign integration with lead scientist Yuming Liu

Update CAMM Filters and Objectives


Video above by Aprameya Mysore is most clearly seen via YouTube.

CAMM Instrumentation Development Book Pages
Coherent laser care notes

CAMM Manuals and Technical Specifications