Horiba FluoroLog Spectrophotometer

Name/Model #
FluoroLog FL3-22
Animal Sciences, 1675 Observatory Drive, Madison, WI 53706

The Fluorolog-3-22 (JY Horiba Inc.) is a spectrofluorometer consisting of a 450 W xenon arc lamp, a double excitation monochromator, a sample compartment with a cuvette holder, a double emission monochromator, and a photomultiplier tube (PMT). A fiber-optic probe can be coupled to the sample compartment such that spectroscopic measurements can be made remotely. Although the spectrofluorometer has been designed for fluorescence spectroscopy, fluorescence (as either an excitation or emission spectrum) as well as reflectance spectra (in synchronous scan mode) can be measured. The adjustable variables are excitation wavelength(s) and emission wavelength(s), excitation and emission slit widths, sampling increment, integration time, and collection geometry (front face versus right angle collection).