LINK is an instrumentation collaboration between the Medical Engineering group in the Morgridge Institute for Research and LOCI investigators that combines quantitative ultrasound (QUS), forwards and backwards scattered second harmonic generation microscopy (SHG), and two optical scattering techniques, enhanced backscatter and optical coherence tomography (EBS and OCT, respectively) to analyze collagen organization in intact animal and human tissues. Paul Campagnola, Kevin Eliceiri, Tim Hall and Jeremy Rogers are collaborating under National Science Foundation Major Research Instrumentation funding and the Morgridge Multiscale Initative on how imaging techniques of different spatial scales can elucidate the role of collagen organization in cancer. 

Class IV Laser: Safety goggles for LINK are located on top of the hooks to the left of the door when you walk in. LINK uses a Class IV Coherent Chameleon Ultra II Ti:Sapph CW laser (SN# GDP.1001H.4175).  Engineers may wish to refer to the American National Standard for Safe Use of Lasers and follow LOCI's General Laser Safety Guidelines.

Safety Goggle Specifications:
LaserVision F22.P1L12.5000
180-315 D LB9 + IR LB4 + M LB6Y (OD 9+)
> 315 - 445 DIRM LB6 (OD 6+)
700 - < 720 DIRM LB4 (OD 4+)
720 - < 740 DIRM LB5 (OD 5+)
740 - < 745 DIRM LB6 (OD 6+)
745 - < 755 D LB6 + IRM LB7 (OD 7+)
755 - 1100 D LB6 + IR LB8 + M LB8Y (OD8+)





"Collagen imaging: Bridging the gap between microscopy and the clinic"

National Science Foundation Major Research Instrumentation Program
Name/Model #
Multiscale Quantitative Ultrasound (QUS), Forward and Backward SHG, Enhanced Backscatter, Optical Coherence Tomography (OCT), Brightfield
271 Animal Sciences, 1675 Observatory Drive, Madison, WI, 53706