Optical Workstation

Name/Model #
Fluorescence Lifetime Imaging (FLIM), Multiphoton Laser Scanning Fluorescence, Brightfield, Optical Ablation
159 Animal Sciences, 1675 Observatory Drive, Madison, WI 53706

The Optical Workstation (OWS) is an inverted multiphoton laser scanning system that uses a Nikon TE300 inverted microscope frame, ASI MS-2000 XYZ stage and WiscScan interface for time-domain fluorescence lifetime imaging (FLIM), live tissue imaging, fluorescence, and second harmonic generation.  

The OWS is a unique multiphoton system with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities.  The primary laser path of the OWS uses a *Spectra-Physics Mai Tai® DeepSee laser tunable between 750-900 nm pumped by a 15W CW 532 nm Millennia laser. A second excitation path contains a Spectra-Physics LSI VSL 337ND nitrogen laser for optical manipulation experiments such as trapping or ablation as described in Review of Scientific Instruments. In addition to the excitation sources, there are two detectors on the OWS.  The fluorescence detector is a Hamamatsu H7422 capable of both intensity and lifetime measurements.  Additionally, a BioRad MRC-1024 photodiode transmission detector from the BioRad MRC-1024 confocal allows for pseudo brightfield imaging. *Spectra-Physics MaiTai DeepSee manual link is to MaiTai HP, which is same system without pulse compensation.

Class IV Laser: Safety goggles for the OWS are 740/780 nm DIR L5 800-1070 nm DIR L7 and are located to the right side of the door hanging on a hook when you walk in. The OWS uses a Class IV Spectra-Physics Mai Tai® DeepSee (#SN 5312) Ti:sapph pulsed laser as its primary source.  Engineers may wish to refer to the American National Standard for Safe Use of Lasers and follow LOCI's General Laser Safety Guidelines.

Scientists and Staff

Adib Keikhosravi and Md Abdul Sagar Kader manage OWS collaborations.

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