BME Lecture: Chuck Dimarzio
BME Lecture, Tong Auditorium
Noon April 22nd, 2019
Electrical and Computer Engineering
Mechanical and Industrial Engineering
Title: Sectioning with Single-View Structured Illumination
Structured illumination microscopy (SIM) is one of three superresolution imaging techniques that won the Nobel Prize in chemistry in 2014. Structured illumination microscopy is also useful for sectioning in scattering materials such as human skin, but most techniques involve multiple images, which make the technique less useful because of motion during the measurement.
The focus of our work is on sectioning to produce three-dimensional images of skin similar to those of confocal reflectance microscopy. We have developed a new technique that can use a single image for sectioning skin instead of the three images usually used in SIM. While a single-image technique is conceptually inferior to the traditional three-image SIM, in the presence of severe heterogeneous refraction and the motion that is inherent in in-vivo imaging, the single image is significantly better. I will discuss the concept and show results on different types of samples.