BME Lecture: Jesse Wilson
Title: Picosecond optical responses of hemes: towards label-free redox contrast of the mitochondrial respiratory chain.
Abstract: The mitochondrial respiratory chain mediates the flow of electrons from NADH and FADH2 to oxygen in order to sustain efficient ATP production. Key electron transport proteins, the cytochromes, have unique optical absorption spectra and picosecond responses to light that report on their redox state. Our goal is to build an optical pump-probe experiment that can measure redox of each of the mitochondrial cytochromes and infer electron flow rates within individual mitochondria in live cells and tissues. Potential applications include characterizing metabolic heterogeneity within the tumor microenvironment and diagnostics for mitochondrial disease.