Laser-scanning microscopes are indispensable tools for biomedical research. Typically, commercial laser-scanning microscopes are controlled by proprietary, purpose-designed electronic hardware and computer software. It is often difficult if not impossible to adapt these systems to new experimental needs, such as incorporating laser microbeam systems for microsurgery or photoactivation. WiscScan was built to address this difficulty. WiscScan combines nearly all the functionality of a laser-scanning microscope/microbeam apparatus into a single software package. This package controls scan waveform generation, signal conditioning, and microbeam pointing. It runs inside a commercial, general-purpose digital signal processor (DSP) residing on a PCI card inside a desktop PC. This host computer loads programs onto the DSP and provides control for microscope focusing and stage movement through a graphical user interface. This all-software approach has allowed us to develop a powerful and flexible development platform for laser-scanning microscopes and has facilitated the incorporation of new functionality such as fluorescence excitation lifetime imaging.

What's new in WiscScan 7.2.2:


Pockels cell can be fully controlled from WiscScan GUI in all systems.

Laser power meter output is shown on WiscScan.

New XYZ generator added to WiscScan.

Automatic white balance controller for QCam was added to WiscScan.

DCC gain controller was added to WiscScan GUI. 

For descriptions of past version updates, see Version History.