Bio-Formats


Overview

Bio-Formats is a standalone Java library for reading and writing life sciences image file formats. It is capable of parsing both pixels and metadata for a large number of formats, as well as writing to several formats. See the table below for a complete list (click the headers to sort, and format names to see all information). For more information on the format ratings, see the Supported Formats page.

Purpose

Bio-Formats's primary purpose is to convert proprietary microscopy data into an open standard called the OME data model, particularly into the OME-TIFF file format. See the Bio-Formats statement of purpose for a thorough explanation and rationale.

For users

Many software packages can use Bio-Formats to read and write microscopy formats:

Libraries and scripting Command line tools
FARSIGHT i3dcore
ImgLib ITK  
Qu for MATLAB Subimager  
Numerical data processing IDL KNIME  
MATLAB VisAD  
Visualization and analysis Bitplane Imaris CellProfiler Comstat2
Endrov Fiji FocalPoint
Graphic Converter

Icy

ImageJ
imago Iqm Macnification
MIPAVVaa3DVisBio
XuvTools

Image servers BISQUE OME server OMERO

For developers

You can use Bio-Formats as a library to easily support these formats in your software.

License

Bio-Formats is open source software licensed under the GNU General Public License (GPL). Alternately, you can obtain a commercial license for Bio-Formats from our commercial partner, Glencoe Software. See the Bio-Formats license page for more information.

Version history and roadmap

Bio-Formats is updated whenever a significant bug fix is made, or whenever a new version of OMERO is released. The version number is three numbers separated by dots; e.g., 4.0.0. See the what's new list for a history of major changes in each release.

The latest stable version of Bio-Formats is 4.3.3. For future development directions, see the 4.4 and 5.0 roadmaps.

Questions

See the Bio-Formats, File Formats and OME-XML and OME-TIFF sections of the OME FAQ for answers to some common questions. Please contact us if you have any questions or problems with Bio-Formats.

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Language: 
Java
Latest Version: 
4.3.3 (2011 October 18)
More Information
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Supported Formats

Export Format Extensions Pixels Openness Presencesort icon Utility
AVI (Audio Video Interleave) .avi
Windows Bitmap .bmp
GIF (Graphics Interchange Format) .gif
JPEG .jpg
PNG (Portable Network Graphics) .png
QuickTime Movie .mov
TIFF (Tagged Image File Format) .tif
Bio-Rad PIC .pic, .raw, .xml
EPS (Encapsulated PostScript) .eps, .epsi, .ps
ICS (Image Cytometry Standard) .ics, .ids
Leica LCS LEI .lei, .tif
MetaMorph Stack (STK) .stk, .nd
Nikon NIS-Elements ND2 .nd2
3i SlideBook .sld
PICT (Macintosh Picture) .pict
Zeiss LSM (Laser Scanning Microscope) 510 .lsm, .mdb
Adobe Photoshop PSD .psd
JPX .jpx
Analyze 7.5 .img, .hdr
Animated PNG .png
Aperio SVS TIFF .svs
DeltaVision .dv, .r3d
DICOM .dcm, .dicom
FITS (Flexible Image Transport System) .fits
Improvision Openlab LIFF .liff
JPEG 2000 .jp2
Leica LAS AF LIF (Leica Image File Format) .lif
Li-Cor L2D .l2d, .tif, .scn
MINC MRI .mnc
MRC (Medical Research Council) .mrc
NIfTI .img, .hdr
Olympus FluoView FV1000 .oib, .oif
Olympus FluoView TIFF .tif
PGM (Portable Gray Map) .pgm
Zeiss AxioVision ZVI (Zeiss Vision Image) .zvi
Bruker MRI
Targa .tga
Photoshop TIFF .tif, .tiff
SPIDER .spi, .stk
IMAGIC .hed, .img
Canon DNG .cr2, .crw
Hitachi S-4800 .txt, .tif, .bmp, .jpg
Amersham Biosciences Gel .gel
Andor Bio-Imaging Division (ABD) TIFF .tif
Bitplane Imaris .ims
FEI .img
Gatan Digital Micrograph .dm3
Hamamatsu Aquacosmos NAF .naf
ImagePro Sequence .seq
Applied Precision CellWorX .htd, .pnl,
InCell 1000 .xdce, .tif
IPLab .ipl
MetaMorph 7.5 TIFF .tiff
μManager .tif, .txt
Minolta MRW .mrw
MNG (Multiple-image Network Graphics) .mng
NRRD (Nearly Raw Raster Data) .nrrd, .nhdr, .raw, .txt
OME-TIFF .ome.tiff
OME-XML .ome
PerkinElmer UltraView .tif, .2, .3, .4, ...
Prairie Technologies TIFF .tif, .xml, .cfg
Amira Mesh .am, .amiramesh, .grey, .hx, .labels
Improvision TIFF .tif
Lambert Instruments FLIM .fli
Hamamatsu HIS .his
Quesant .afm
Molecular Imaging .stp
Topometrix .tfr, .ffr, .zfr, .zfp, .2fl
JEOL .dat, .img, .par
Seiko .xqd, .xqf
Oxford Instruments .top
LEO .sxm
Burleigh .img
Unisoku .dat, .hdr
BD Pathway .exp, .tif
RHK .sm2, .sm3
SM Camera
SBIG
JPK .jpk
Gatan Digital Micrograph 2 .dm2
WA-TOP .wat
ECAT7 .v
FEI TIFF .tiff
Varian FDF .fdf
SimplePCI & HCImage TIFF .tiff
Nikon Elements TIFF .tiff
InCell 3000 .frm
Trestle .tif, .sld, .jpg
Olympus SIS TIFF .tiff
Hamamatsu VMS .vms
Hamamatsu .ndpi .ndpi
cellSens VSI .vsi
INR .inr
Volocity Library Clipping .acff
Kodak BIP .bip
Axon Raw Format .arf
Alicona 3D .al3d
Becker & Hickl SPCImage .sdt
Cellomics .c01
Evotec/PerkinElmer Opera Flex .flex
ImagePro Workspace .ipw
Improvision Openlab Raw .raw
IPLab-Mac .ipm
Khoros VIFF (Visualization Image File Format) Bitmap .xv
LIM (Laboratory Imaging/Nikon) .lim
NEF (Nikon Electronic Format) .nef, .tif
Olympus CellR/APL .apl, .mtb, .tnb, .tif, .obsep
PCX (PC Paintbrush) .pcx
SimplePCI & HCImage .cxd
TillPhotonics TillVision .vws
VisiTech XYS .xys, .html
MIAS (Maia Scientific) .tif
Nikon EZ-C1 TIFF .tiff
Olympus ScanR .xml, .dat, .tif
Bio-Rad Gel .1sc
Imacon .fff
UBM .pr3
VG SAM .dti
Perkin Elmer Densitometer .pds
AIM .aim
Volocity .mvd2
Text .txt